Product info:

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Alternative names:

DDDDK; DDDDK tag; DDDDK-tag

Species reactivity:

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Host:

Rabbit

Cellular localisation:

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Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to DDDDK tag.

Positive control:

293T

AA Sequence:

DYKDDDDK

Purification:

Affinity purification

Molecular weight:

Observed MW: 50KDa

Form:

Liquid

Applications:

IP (Oryza sativa, Homo sapiens, Danio rerio) WB (Mus musculus, Homo sapiens, Sus scrofa) ChIP (Homo sapiens) IF (Homo sapiens, Sus scrofa) Co-IP (Homo sapiens)

Dilution:

WB 1:1000 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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