Product info:

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Alternative names:

ACTB; BRWS1; PS1TP5BP1; beta actin; actin

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, cytoskeleton

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human β-Actin (NP_001092.1).

Positive control:

HeLa, MCF7, A-431, NIH/3T3, C6, Mouse testis, Mouse kidney, Rat testis

AA Sequence:

MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Purification:

Affinity purification

Molecular weight:

Calculated MW: 41kDa Observed MW: 42kDa

Form:

Liquid

Applications:

WB (MSC cells, Mus musculus, Arabidopsis thaliana, Rattus norvegicus, Homo sapiens, Tree, Flowers, flounder, lamprey, Melodinus cochinchinensis, Sus scrofa, Sus scrofa , Crassostrea gigas, Gallus gallus) IF (Homo sapiens, Rattus norvegicus, Homo sapiens, Mus musculus) IP (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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