Product info:

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Alternative names:

His; His tag; His-tag

Species reactivity:

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Host:

Mouse

Cellular localisation:

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Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to His tag.

Positive control:

293T

AA Sequence:

HHHHHH

Purification:

Affinity purification

Molecular weight:

Observed MW: 35KDa

Form:

Liquid

Applications:

WB (Danio rerio, Homo sapiens, Arabidopsis thaliana, Other, Mus musculus, Oryctolagus cuniculus, Aspergillus nidulans, Oryza sativa, Cotton bollworms, Escherichia coli, Cyprinus carpio, Helicoverpa armigera, Ictalurus punctatus, Sus scrofa, Chlamys Farreri, Zooxanthella, Brachyura, Crassostrea gigas, Plasmodium, D. folliculorum and D. brevis adults) IF (Homo sapiens, Mus musculus, Rattus norvegicus, Danio rerio) IP (Homo sapiens, Cyprinus carpio, Mus musculus) ELISA (Cynoglossus semilaevis, Mus musculus, Rattus norvegicus, Oyster) Co-IP (Helicoverpa armigera) IHC (Homo sapiens)

Dilution:

WB 1:2000 - 1:5000 IF 1:50 - 1:200 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com