Product info:

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes. Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Alternative names:

RFP; RFP tag; RFP-tag

Species reactivity:

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Host:

Mouse

Cellular localisation:

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Isotype:

IgG

Immunogen:

Recombinant protein of RFP-Tag

Positive control:

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AA Sequence:

MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHHLFL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 27kDa Observed MW: Refer to Figures

Form:

Liquid

Applications:

WB (Helicoverpa armigera, Cotton bollworms, Nicotiana benthamiana, Homo sapiens) Co-IP (Helicoverpa armigera) IP (Gallus gallus) ChIP (Homo sapiens)

Dilution:

WB 1:2000 - 1:5000 IF 1:50 - 1:200 IP 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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