Product info:

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

Alternative names:

BRAF; B-RAF1; B-raf; BRAF1; NS7; RAFB1

Species reactivity:

Human, Rat

Host:

Rabbit

Cellular localisation:

Cell membrane, Cytoplasm, Nucleus

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around S445 of human BRAF (NP_004324.2).

Positive control:

Raji

AA Sequence:

RDSSD

Purification:

Affinity purification

Molecular weight:

Calculated MW: 84kDa Observed MW: 100kDa

Form:

Liquid

Applications:

WB (Rattus norvegicus, Gallus gallus)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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