Product info:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Alternative names:

H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A

Species reactivity:

Human, Mouse, Rat, Other (Wide Range)

Host:

Rabbit

Cellular localisation:

Chromosome, Nucleus

Isotype:

IgG

Immunogen:

A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Positive control:

HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 15kDa Observed MW: 17KDa

Form:

Liquid

Applications:

WB (Human cells, HEK293T, Mouse Bone marrow stromal cells, Homo sapiens, Mus musculus, Rattus norvegicus, Oryza sativa) ChIP (Plant samples, Mouse primary calvarial cells, Mus musculus, Oryza sativa, Rattus norvegicus) IF (HEK293T, Mouse E16.5 neocortex, Raw264.7, Mus musculus, Oryza sativa, Rattus norvegicus, Homo sapiens) ChIP-Seq (Zea mays) IP (Homo sapiens) IHC (Mus musculus, Rattus norvegicus, Bombyx mori)

Dilution:

DB 1:500 - 1:2000 WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIP-seq 1:20 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com