Product info:

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternative names:

SF3B1; Hsh155; MDS; PRP10; PRPF10; SAP155; SF3b155

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Nucleus speckle

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2).

Positive control:

NIH/3T3, PC-12

AA Sequence:

AETPR

Purification:

Affinity purification

Molecular weight:

Calculated MW: 16kDa/145kDa Observed MW: 155kDa

Form:

Liquid

Applications:

WB (Homo sapiens, Mus musculus)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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