Product info:

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]

Alternative names:

ESPL1; ESP1; SEPA; separin

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus

Isotype:

IgG

Immunogen:

A synthetic phosphorylated peptide around S1126 of human ESPL1 (NP_036423.4).

Positive control:

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AA Sequence:

NSSPV

Purification:

Affinity purification

Molecular weight:

Calculated MW: 197kDa/233kDa Observed MW: Refer to Figures

Form:

Liquid

Applications:

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Dilution:

WB 1:500 - 1:2000 IF 1:20 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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