Product info:

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Alternative names:

HMG-1; HMG1; HMG3; SBP-1; HMGB1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).

Positive control:

HeLa, MCF7

AA Sequence:

SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEE

Purification:

Affinity purification

Molecular weight:

Calculated MW: 24kDa Observed MW: 29KDa

Form:

Liquid

Applications:

WB (Mouse brain, Mouse spinal cord, Homo sapiens, Mus musculus) IHC (HeLa, Rattus norvegicus, Mus musculus) IF (Homo sapiens, Mus musculus, Radula lindenbergiana Gott. ex Hartm) IP (Mus musculus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com