Product info:

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternative names:

IL18; IGIF; IL-18; IL-1g; IL1F4

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Secreted

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).

Positive control:

Recombinant Human IL-18 Protein, HeLa, Raw264.7, Rat brain

AA Sequence:

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

Purification:

Affinity purification

Molecular weight:

Calculated MW: 21kDa/22kDa Observed MW: 18KDa/22KDa

Form:

Liquid

Applications:

WB (N9 cells, HUVEC cells, Mus musculus, Homo sapiens) IF (Mus musculus) IHC (Mus musculus)

Dilution:

WB 1:500 - 1:2000 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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