Product info:

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Alternative names:

TOP2A; TOP2; TP2A

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus, nucleoplasm

Isotype:

IgG

Immunogen:

A synthetic peptide of human DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A))

Positive control:

HepG2

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 174kDa/177kDa/178kDa/182kDa Observed MW: 190KDa

Form:

Liquid

Applications:

WB (H460, A549, Homo sapiens) IF (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50

Storage buffer:

Buffer: PBS with 0.02% sodium azide, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com