Product info:

S1 region attaches the virion to the cell membrane by interacting with host ACE2, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Alternative names:

N/A

Species reactivity:

HCoV-NL63

Host:

Rabbit

Cellular localisation:

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Isotype:

IgG

Immunogen:

Recombinant protein of HCoV-NL63 Spike S2.

Positive control:

Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag)

AA Sequence:

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Purification:

Affinity purification

Molecular weight:

Calculated MW: 149kDa Observed MW: 160KDa

Form:

Liquid

Applications:

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Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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