Product info:

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

Alternative names:

CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1; PKM

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of human PKM2 (NP_002645.3).

Positive control:

Jurkat, HeLa, A-549

AA Sequence:

AFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAV

Purification:

Affinity purification

Molecular weight:

Calculated MW: 56kDa/57kDa/58kDa Observed MW: 62kDa

Form:

Liquid

Applications:

WB (NCI-N87 cells, Human Bladder Cancer, Human cells, U251, U87MG, Homo sapiens) IP (Homo sapiens) IHC (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IF 1:20 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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