Product info:

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).

Alternative names:

ATG12; APG12; APG12L; FBR93; HAPG12; Apg12

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 1-74 of human ATG12 (NP_001264712.1).

Positive control:

HCT116, C6, Rat testis

AA Sequence:

MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIYLCESVLCSFPRPRSWNSL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 7kDa/15kDa Observed MW: 55KDa

Form:

Liquid

Applications:

IF (Mus musculus)

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com