Product info:

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Alternative names:

CFL1; CFL; HEL-S-15; cofilin; cofilin-1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell projection, Cytoplasm, Cytoplasmic side, Nucleus matrix, Peripheral membrane protein, cytoskeleton, lamellipodium membrane, ruffle membrane

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFL1 (NP_005498.1).

Positive control:

HeLa, A-549, Jurkat, 293T, Mouse brain, Mouse liver, Mouse kidney, Rat brain, Rat ovary

AA Sequence:

MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLV

Purification:

Affinity purification

Molecular weight:

Calculated MW: 18kDa Observed MW: 19KDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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