Product overview:

RASA1 (GAP ) mouse monoclonal antibody, clone LBI4E2

Field of research:

Axon guidance, MAPK signaling pathway

Summary:

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]

Alternative names:

CM-AVM; CMAVM; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

RASA1

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human RASA1(NP_002881) produced in HKE293T cell.

Clone:

LBI4E2

Conjugation:

Unconjugated

Gene name:

RAS p21 protein activator 1

Symbol:

RASA1

Pathway:

Axon guidance, MAPK signaling pathway

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IF 1:100

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com