Product overview:

DBT mouse monoclonal antibody, clone LBI1G2

Field of research:

Metabolic pathways, Valine, leucine and isoleucine degradation

Summary:

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternative names:

BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

DBT

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.

Clone:

LBI1G2

Conjugation:

Unconjugated

Gene name:

dihydrolipoamide branched chain transacylase E2

Symbol:

DBT

Pathway:

Metabolic pathways, Valine, leucine and isoleucine degradation

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, FLOW 1:100

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com