Product overview:

ARFGAP1 mouse monoclonal antibody, clone LBI1H4

Field of research:

Endocytosis

Summary:

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternative names:

ARF1GAP; HRIHFB2281

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

ARFGAP1

Isotype:

IgG2b

Immunogen:

Full length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.

Clone:

LBI1H4

Conjugation:

Unconjugated

Gene name:

ADP ribosylation factor GTPase activating protein 1

Symbol:

ARFGAP1

Pathway:

Endocytosis

Function:

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Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.7 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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