Product overview:

Anti-EIF2S1 mouse monoclonal antibody, clone LBI5D10

Field of research:

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Summary:

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]). [supplied by OMIM]

Alternative names:

EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

EIF2S1

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.

Clone:

LBI5D10

Conjugation:

Unconjugated

Gene name:

eukaryotic translation initiation factor 2 subunit alpha

Symbol:

EIF2S1

Pathway:

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Function:

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Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.65 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:50, IF 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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