Product overview:

Anti-NIT2 mouse monoclonal antibody, clone LBI5A3

Field of research:

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Summary:

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle

Alternative names:

HEL-S-8a

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

NIT2

Isotype:

IgG2a

Immunogen:

Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.

Clone:

LBI5A3

Conjugation:

Unconjugated

Gene name:

nitrilase family member 2

Symbol:

NIT2

Pathway:

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Function:

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Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.95 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:50, IF 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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