Product overview:

HNRNPF (hnRNP F) mouse monoclonal antibody, clone LBI5F5

Field of research:

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Summary:

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Alternative names:

HNRPF; mcs94-1; OK/SW-cl.23

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

HNRNPF

Isotype:

IgG2a

Immunogen:

Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.

Clone:

LBI5F5

Conjugation:

Unconjugated

Gene name:

heterogeneous nuclear ribonucleoprotein F

Symbol:

HNRNPF

Pathway:

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Function:

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Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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