Product overview:

Anti-CYP2E1 (Cytochrome P450 2E1) mouse monoclonal antibody, clone LBI5F11

Field of research:

Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450

Summary:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Alternative names:

CPE1; CYP2E; P450-J; P450C2E

Species reactivity:

Human

Host:

Mouse

Target:

CYP2E1

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.

Clone:

LBI5F11

Conjugation:

Unconjugated

Gene name:

cytochrome P450 family 2 subfamily E member 1

Symbol:

CYP2E1

Pathway:

Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450

Function:

Druggable Genome, P450, Transmembrane

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:500, IHC 1:50, IF 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com