Product overview:

Patched1 (Patched) mouse monoclonal antibody, clone LBI5C7

Field of research:

Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

Summary:

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

Alternative names:

BCNS; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

PTCH1

Isotype:

IgG2b

Immunogen:

Human recombinant protein fragment corresponding to amino acids 122-436 of human PTCH1 (NP_000255) produced in E.coli.

Clone:

LBI5C7

Conjugation:

Unconjugated

Gene name:

patched 1

Symbol:

PTCH1

Pathway:

Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

Function:

Druggable Genome, ES Cell Differentiation/IPS, Transmembrane

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.2 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:500, IHC 1:50, IF 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com