Product overview:

Anti-Notch1 mouse monoclonal antibody, clone LBI3E12

Field of research:

Dorso-ventral axis formation, Notch signaling pathway, Prion diseases

Summary:

Notch1 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development

Alternative names:

AOS5; AOVD1; hN1; TAN1

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

NOTCH1

Isotype:

IgG2b

Immunogen:

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1

Clone:

LBI3E12

Conjugation:

Unconjugated

Gene name:

notch receptor 1

Symbol:

NOTCH1

Pathway:

Dorso-ventral axis formation, Notch signaling pathway, Prion diseases

Function:

Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.2 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB: 1:1000; IHC 1;50; RPPA 1:75; IF: 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com