Product overview:

DFF45 (ICAD) mouse monoclonal antibody, clone LBI3F12

Field of research:

Apoptosis

Summary:

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternative names:

DFF-45; DFF1; ICAD

Species reactivity:

Human, Monkey

Host:

Mouse

Target:

DFFA

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human DFFA (NP_004392) produced in E.coli.

Clone:

LBI3F12

Conjugation:

Unconjugated

Gene name:

DNA fragmentation factor subunit alpha

Symbol:

DFFA

Pathway:

Apoptosis

Function:

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Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.2 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:200~500, IF 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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