Product overview:

TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone LBI5H2

Field of research:

Gap junction, Pathogenic Escherichia coli infection

Summary:

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules.

Alternative names:

beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

TUBB3

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-150 of human TUBB3 (NP_006077) produced in E.coli.

Clone:

LBI5H2

Conjugation:

Unconjugated

Gene name:

tubulin beta 3 class III

Symbol:

TUBB3

Pathway:

Gap junction, Pathogenic Escherichia coli infection

Function:

Druggable Genome, ES Cell Differentiation/IPS

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:1000~2000

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com