Product overview:

Anti-SHH (Sonic Hedgehog) mouse monoclonal antibody, clone LBI3A2

Field of research:

Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

Summary:

SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.

Alternative names:

HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

SHH

Isotype:

IgG2a

Immunogen:

Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH

Clone:

LBI3A2

Conjugation:

Unconjugated

Gene name:

sonic hedgehog signaling molecule

Symbol:

SHH

Pathway:

Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

Function:

Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.12 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB: 1:2000, IF (1:100)

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com