Product overview:

OCT4 mouse monoclonal antibody, clone LBI9B7

Field of research:

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Summary:

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Alternative names:

Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4

Species reactivity:

Human

Host:

Mouse

Target:

44838

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human POU5F1 (NP_002692) produced in E.coli.

Clone:

LBI9B7

Conjugation:

Unconjugated

Gene name:

POU class 5 homeobox 1

Symbol:

44838

Pathway:

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Function:

Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.25 mg/ml

Form:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:5000, IHC 1:50, IF 1:100

Storage buffer:

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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