Product overview:

HGF mouse monoclonal antibody, clone LBI1E5

Field of research:

Cytokine-cytokine receptor interaction, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma

Summary:

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Alternative names:

DFNB39; F-TCF; HGFB; HPTA; SF

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

HGF

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 200-494 of human HGF(NP_000592) produced in E.coli.

Clone:

LBI1E5

Conjugation:

Unconjugated

Gene name:

hepatocyte growth factor

Symbol:

HGF

Pathway:

Cytokine-cytokine receptor interaction, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma

Function:

Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com