Product overview:

MCM2 mouse monoclonal antibody, clone LBI8A11

Field of research:

Cell cycle, DNA replication

Summary:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Alternative names:

BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

MCM2

Isotype:

IgG2b

Immunogen:

Full length human recombinant protein of human MCM2 (NP_004517) produced in HEK293T cell.

Clone:

LBI8A11

Conjugation:

Unconjugated

Gene name:

minichromosome maintenance complex component 2

Symbol:

MCM2

Pathway:

Cell cycle, DNA replication

Function:

Druggable Genome, Stem cell - Pluripotency, Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com