Product overview:

PCNA mouse monoclonal antibody, clone LBI1A5

Field of research:

Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

Summary:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Alternative names:

ATLD2

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

PCNA

Isotype:

IgG2b

Immunogen:

Full length human recombinant protein of human PCNA (NP_002583) produced in E.coli.

Clone:

LBI1A5

Conjugation:

Unconjugated

Gene name:

proliferating cell nuclear antigen

Symbol:

PCNA

Pathway:

Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com