Product overview:

FEN1 mouse monoclonal antibody, clone LBI3A8

Field of research:

Base excision repair, DNA replication, Non-homologous end-joining

Summary:

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Alternative names:

FEN-1; MF1; RAD2

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

FEN1

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 117-380 of human FEN1 (NP_004102) produced in E.coli.

Clone:

LBI3A8

Conjugation:

Unconjugated

Gene name:

flap structure-specific endonuclease 1

Symbol:

FEN1

Pathway:

Base excision repair, DNA replication, Non-homologous end-joining

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com