Product overview:

TYMS mouse monoclonal antibody, clone LBI1B1

Field of research:

Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism

Summary:

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

Alternative names:

HST422; TMS; TS

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

TYMS

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human TYMS (NP_001062) produced in E.coli.

Clone:

LBI1B1

Conjugation:

Unconjugated

Gene name:

thymidylate synthetase

Symbol:

TYMS

Pathway:

Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com