Product overview:

VIM (Vimentin) mouse monoclonal antibody, clone LBI7F5

Field of research:

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Summary:

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. [provided by RefSeq, Jun 2009]

Alternative names:

CTRCT30; HEL113

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

VIM

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli.

Clone:

LBI7F5

Conjugation:

Unconjugated

Gene name:

vimentin

Symbol:

VIM

Pathway:

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Function:

ES Cell Differentiation/IPS

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:200 - 1:1000

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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