Product overview:

PYCARD (TMS1) mouse monoclonal antibody, clone LBI3E9

Field of research:

Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

Summary:

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternative names:

ASC; CARD5; TMS; TMS-1; TMS1

Species reactivity:

Human

Host:

Mouse

Target:

PYCARD

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.

Clone:

LBI3E9

Conjugation:

Unconjugated

Gene name:

PYD and CARD domain containing

Symbol:

PYCARD

Pathway:

Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:500~2000, IHC 1:150, FLOW 1:100

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com