Product overview:

GCLC mouse monoclonal antibody, clone LBI1A3

Field of research:

Glutathione metabolism, Metabolic pathways

Summary:

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction. [provided by RefSeq, Oct

Alternative names:

GCL; GCS; GLCL; GLCLC

Species reactivity:

Human, Monkey, Mouse, Rat

Host:

Mouse

Target:

GCLC

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.

Clone:

LBI1A3

Conjugation:

Unconjugated

Gene name:

glutamate-cysteine ligase catalytic subunit

Symbol:

GCLC

Pathway:

Glutathione metabolism, Metabolic pathways

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:200~1000, IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com