Product overview:

Carrier-free (BSA/glycerol-free) PHGDH mouse monoclonal antibody, clone LBI5C4

Field of research:

Glycine, serine and threonine metabolism, Metabolic pathways

Summary:

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]

Alternative names:

3-PGDH; 3PGDH; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PHGDHD; SERA

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

PHGDH

Isotype:

IgG2a

Immunogen:

Full length human recombinant protein of human PHGDH (NP_006614) produced in HEK293T cell.

Clone:

LBI5C4

Conjugation:

Unconjugated

Gene name:

Homo sapiens phosphoglycerate dehydrogenase (PHGDH), mRNA.

Symbol:

PHGDH

Pathway:

Glycine, serine and threonine metabolism, Metabolic pathways

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com