Product overview:

Carrier-free (BSA/glycerol-free) NR3C1 mouse monoclonal antibody, clone LBI7A3

Field of research:

Neuroactive ligand-receptor interaction

Summary:

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Alternative names:

GCCR; GCR; GCRST; GR; GRL

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

NR3C1

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-265 of human NR3C1(NP_000167) produced in E.coli.

Clone:

LBI7A3

Conjugation:

Unconjugated

Gene name:

Homo sapiens nuclear receptor subfamily 3 group C member 1 (NR3C1), transcript variant 1, mRNA.

Symbol:

NR3C1

Pathway:

Neuroactive ligand-receptor interaction

Function:

Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com