Product overview:

Carrier-free (BSA/glycerol-free) NFS1 mouse monoclonal antibody, clone LBI5D1

Field of research:

Thiamine metabolism

Summary:

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov

Alternative names:

COXPD52; HUSSY-08; IscS; NIFS

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

NFS1

Isotype:

IgG2a

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-299 of human NFS1(NP_066923) produced in E.coli.

Clone:

LBI5D1

Conjugation:

Unconjugated

Gene name:

Homo sapiens NFS1 cysteine desulfurase (NFS1), transcript variant 1, mRNA; nuclear gene for mitochondrial product.

Symbol:

NFS1

Pathway:

Thiamine metabolism

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500, IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com