Product overview:

Carrier-free (BSA/glycerol-free) MLH1 mouse monoclonal antibody, clone LBI1C1

Field of research:

Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

Summary:

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined. [provided by RefSeq, Nov 2009]

Alternative names:

COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

MLH1

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 299-527 of human MLH1(NP_000240) produced in E.coli.

Clone:

LBI1C1

Conjugation:

Unconjugated

Gene name:

Homo sapiens mutL homolog 1 (MLH1), transcript variant 1, mRNA.

Symbol:

MLH1

Pathway:

Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com