Product overview:

CD5 mouse monoclonal antibody, clone UMLBI9

Field of research:

Hematopoietic cell lineage

Summary:

CD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. B-1 cells have limited diversity of theirB-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 is a good immunohistochemical marker for T-cells. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia, hairy cell leukaemia, and mantle cell lymphoma cells. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukaemia, while relatively rare, is associated with a poor prognosis.

Alternative names:

LEU1; T1

Species reactivity:

Human

Host:

Mouse

Target:

CD5

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.

Clone:

UMLBI9

Conjugation:

Unconjugated

Gene name:

Homo sapiens CD5 molecule (CD5), transcript variant 1, mRNA.

Symbol:

CD5

Pathway:

Hematopoietic cell lineage

Function:

Druggable Genome, Transmembrane

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

0.5~1.0 mg/ml (Lot Dependent)

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:500, IHC 1:100, IF 1:100, FLOW 1:100, IF 1:100

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com