Product overview:

MLH1 mouse monoclonal antibody, clone LBI4H4

Field of research:

Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

Summary:

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Alternative names:

COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

MLH1

Isotype:

IgG2a

Immunogen:

Human recombinant protein fragment corresponding to amino acids 339-558 of human MLH1 (NP_000240) produced in E.coli.

Clone:

LBI4H4

Conjugation:

Unconjugated

Gene name:

mutL homolog 1

Symbol:

MLH1

Pathway:

Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

IHC 1:1000

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com