Product overview:

CAD mouse monoclonal antibody,clone LBI10A3

Field of research:

Alanine, aspartate and glutamate metabolism, Metabolic pathways, Pyrimidine metabolism

Summary:

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Alternative names:

CDG1Z; DEE50; EIEE50; GATD4

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

CAD

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1918-2225 of human CAD (NP_004332) produced in E.coli.

Clone:

LBI10A3

Conjugation:

Unconjugated

Gene name:

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

Symbol:

CAD

Pathway:

Alanine, aspartate and glutamate metabolism, Metabolic pathways, Pyrimidine metabolism

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:250~500

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com