Product overview:

SMC1A mouse monoclonal antibody,clone LBI5A1

Field of research:

Cell cycle, Oocyte meiosis

Summary:

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Alternative names:

CDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

SMC1A

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 889-1016 of human SMC1A (NP_006297) produced in E.coli.

Clone:

LBI5A1

Conjugation:

Unconjugated

Gene name:

structural maintenance of chromosomes 1A

Symbol:

SMC1A

Pathway:

Cell cycle, Oocyte meiosis

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:500, IHC 1:500

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com