Product overview:

Carrier-free (BSA/glycerol-free) ANAPC2 mouse monoclonal antibody, clone LBI1H8

Field of research:

Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

Summary:

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq]

Alternative names:

APC2

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

ANAPC2

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.

Clone:

LBI1H8

Conjugation:

Unconjugated

Gene name:

Homo sapiens anaphase promoting complex subunit 2 (ANAPC2), mRNA.

Symbol:

ANAPC2

Pathway:

Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000, IF 1:100, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com