Product overview:

Carrier-free (BSA/glycerol-free) COASY mouse monoclonal antibody, clone LBI2H1

Field of research:

Metabolic pathways, Pantothenate and CoA biosynthesis

Summary:

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM]

Alternative names:

DPCK; NBIA6; NBP; pOV-2; PPAT; UKR1

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

COASY

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.

Clone:

LBI2H1

Conjugation:

Unconjugated

Gene name:

Homo sapiens Coenzyme A synthase (COASY), transcript variant 1, mRNA.

Symbol:

COASY

Pathway:

Metabolic pathways, Pantothenate and CoA biosynthesis

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:150, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com