Product overview:

Carrier-free (BSA/glycerol-free) LIMK1 mouse monoclonal antibody, clone LBI8C8

Field of research:

Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

Summary:

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alternative names:

LIMK; LIMK-1

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

LIMK1

Isotype:

IgG2a

Immunogen:

Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.

Clone:

LBI8C8

Conjugation:

Unconjugated

Gene name:

Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1, mRNA.

Symbol:

LIMK1

Pathway:

Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

Function:

Druggable Genome, Protein Kinase

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:1000, IHC 1:150, IF 1:100, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com