Product overview:

Carrier-free (BSA/glycerol-free) ATP6V1B1 mouse monoclonal antibody, clone LBI1H6

Field of research:

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Summary:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq]

Alternative names:

ATP6B1; RTA1B; VATB; VMA2; VPP3

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

ATP6V1B1

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human ATP6V1B1 (NP_001683) produced in HEK293T cell.

Clone:

LBI1H6

Conjugation:

Unconjugated

Gene name:

Homo sapiens ATPase H+ transporting V1 subunit B1 (ATP6V1B1), mRNA.

Symbol:

ATP6V1B1

Pathway:

Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com