Product overview:

Carrier-free (BSA/glycerol-free) ARFGAP1 mouse monoclonal antibody, clone LBI1F9

Field of research:

Endocytosis

Summary:

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternative names:

ARF1GAP; HRIHFB2281

Species reactivity:

Human, Monkey, Mouse, Rat

Host:

Mouse

Target:

ARFGAP1

Isotype:

IgG2b

Immunogen:

Full length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.

Clone:

LBI1F9

Conjugation:

Unconjugated

Gene name:

Homo sapiens ADP ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2, mRNA.

Symbol:

ARFGAP1

Pathway:

Endocytosis

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000, IF 1:100, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com