Product overview:

Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody, clone LBI3B5

Field of research:

Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

Summary:

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]

Alternative names:

hRPB25; hsRPB5; RPABC1; RPB5; XAP4

Species reactivity:

Human, Monkey, Mouse, Rat, Dog

Host:

Mouse

Target:

POLR2E

Isotype:

IgG2a

Immunogen:

Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.

Clone:

LBI3B5

Conjugation:

Unconjugated

Gene name:

Homo sapiens RNA polymerase II, I and III subunit E (POLR2E), transcript variant 1, mRNA.

Symbol:

POLR2E

Pathway:

Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

Function:

Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000, IHC 1:150, FLOW 1:100, IF: 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com